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. 2020 Jul 2;10:10835. doi: 10.1038/s41598-020-67783-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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VDR agonist activity on DENV E protein and VDR expression assessed by immunofluorescence assay. Mock or DENV 2 infected HEK293T/17 cells were treated with 0.01% DMSO or 10 μM of VDR agonists (ZD-1, ZD-2, ZD-3, ZD-5 and ZD-6). After 24 h of treatment, cells were processed under the standard procedure of immunofluorescence assay. DENV E protein (green) and VDR (red) were detected using specific antibodies. Nuclei were stained with DAPI (blue). All the signal was observed under a LSM 800 w Airyscan (ZEISS, Oberkochen, Germany) confocal microscope with 60X magnification.