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. 2020 Jul 2;10:10835. doi: 10.1038/s41598-020-67783-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antiviral activity of a VDR agonist against other flaviviruses. HEK293T/17 cells were infected with other flaviviruses, including DENV 1 (strain 16007), DENV 3 (strain 16562), DENV 4 (strain 1036), JEV (strain BJ1) and ZIKV (strain SV0010/15). Infected cells were incubated with 10 μM ZD-6 in complete medium and at 24 h.p.i., virus titer was determined by standard plaque assay. p value; *< 0.05, **< 0.01, ***< 0.001 for significance. The experiment was performed independently in triplicate with duplicate in plaque assay.