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VDR regulated gene expression by qRT-PCR. HEK293T/17 cells were treated with 10 µM VDR agonists for 24 h. RNA was isolated and gene expression levels determined using qRT-PCR. The relative gene expression of VDR, CYP27B1 and CYP24A1 was normalized against a housekeeping gene (actin) and untreated cells (0.01%DMSO), respectively. p value; *< 0.05, **< 0.01, ***< 0.001 for significance. All experiments were performed in triplicate.
