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. 2020 Jul 2;10:10893. doi: 10.1038/s41598-020-67449-w

Figure 1.

Figure 1

Validation of qPCR primers. (A) Melting curve analysis for testing primer specificity. A single peak indicates a single PCR-product. (B) qPCR products of each target gene were run on a 2% agarose gel. Each gene shows a single fluorescent band at the expected amplicon size (Table 1). The gel was detected with ChemiDoc MP Imaging System (BioRad), exported as a TIFF image, inverted and cropped to show the relevant gel area. See Supplementary Data 1 for the unedited image. (bp = base pairs).