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. 2020 Jul 2;10:10880. doi: 10.1038/s41598-020-67811-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MA plots of axenic versus conventionally reared mosquito genes. The beta value in Sleuth indicates the estimated fold change for each gene. Each dot is representative of one gene; grey dots represent no significant differences between axenic and conventionally raised groups. Red dots represent genes that were either enriched (above the x-axis) or depleted (below the x-axis) and were considered biologically significant in abundance. The four treatment groups investigated were midguts blood-fed (MBF) (a), midguts sugar-fed (MSF) (b), carcasses blood-fed (CBF) (c) and carcasses sugar-fed (CSF) (d).