Figure 3.

Western-blot analysis of PC3 (A) (entire blot shown in Figure S1), MCF-7 (B), and HCT116 (C) cell lysates incubated with DEVD probe or CS1 probe at 1 and 10 μM for 1 hour, at 37°C, shaking (500 rpm). Caspase-3 and caspase-7 activation was induced with previously validated treatments (Figure 2). Untreated cells were also incubated with the two probes as negative controls. SDS-PAGE was run in 15 % poly-acrylamide gels and, following protein transfer in immobilon membranes, the blots were probed with streptavidin-HRP to visualise caspase-3/−7 labelling. Equal loading was ensured by b-tubulin immuno-blot from the same gel.