Extended Data Fig. 5. Echocardiography parameters.

a, left ventricular internal dimension in diastole (LVIDd); b, left ventricular internal dimension in systole (LVIDs), echocardiogram measurements, comparing PDK4 KO and the control αMHC-MerCreMer (MCM) at different time points (n=5 biologically independent mice per group). Data are represented as the mean±s.d. Analyses were performed by unpaired two-tailed Student’s t-test to compare MCM vs PDK4 KO. Paired two-tailed Student’s t-test was used to compare two time points within the same mouse (6 months post MI versus 1 week post MI). c, LVDd; d, LVIDs echocardiogram measurements, comparing uninjured versus myocardial infarction (MI) MCM and PDK4 KO groups (n=4 biologically independent mice for uninjured and n=5 biologically independent mice for MI groups). Data are represented as the mean±s.e.m. Analyses were performed by Two-way ANOVA followed by Tukey’s multiple comparisons test, with individual variance computed for each comparison. ns, not significant. e, Trichrome staining of control MCM and PDK4 KO uninjured hearts, 6 months post Tamoxifen injection. Images are representative of three independently performed experiments, with similar results.