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. Author manuscript; available in PMC: 2020 Jul 2.
Published in final edited form as: Ann Integr Mol Med. 2020;2(1):90–114. doi: 10.33597/aimm.02-1007

Figure 1: Projected stages of the chimeric pathway of RdRp-facilitated, antisense RNA-mediated amplification of mammalian mRNA.

Figure 1:

Top panel: Conventional, genome-originated mRNA molecule. Bottom panel: Projected stages of antisense RNA-mediated mRNA amplification. Boxed line – sense strand RNA. Single line – antisense strand RNA. “AUG” – functional translation initiation codon (could be other than “AUG”). “TCE”– 3’-terminal complementary element; “ICE”– internal complementary element, both on the antisense RNA strand. Yellow circle – helicase/modifying activity complex. Blue lines (both single and boxed) – RNA strand modified and separated from its complement by a helicase complex. Red arrowhead – position of cleavage of the chimeric intermediate. Step 1: Synthesis of antisense strand; step 2: Strand separation; step 3: Folding of antisense strand into self-priming configuration; step 4: Extension of self-primed antisense RNA; step 5: Strand separation; step 6: Cleavage of the chimeric intermediate; step 7: End-products of amplification. Note that chimeric RNA end product retains the intact coding capacity of conventional mRNA.