Skip to main content
. 2020 Jul 2;15(7):e0235553. doi: 10.1371/journal.pone.0235553

Fig 5. Trail upregulation in aneurysm tissue of AngII-infused ApoE-/-Opg-/- mice.

Fig 5

(A) Representative immunofluorescence images of aortic tissue sections from AngII-infused mice stained with anti-Trail (green) antibody. Scale bars indicate 100 μm. Areas selected by the white box (dotted line) in the Aneurysm group are magnified below. (B) Magnified immunofluorescence images of aortic tissue sections from the AngII-infused Aneurysm group stained with anti-Trail (green; a, f), anti-SMA (green; b, g), anti-vimentin (red; c, h), anti-Ki67 (red; d, i), and anti-F4/80 (red; e, j) antibodies in ApoE-/-Opg+/+ and ApoE-/-Opg-/- mice. Nuclei were stained with DAPI (blue; c-DAPI, h-DAPI). Scale bars indicate 25 μm. Medial layer (m), adventitial layer (a). The two arrowheads show borders of the medial layer. The white dotted line in the magnified panels indicates the border of the medial and adventitial layers. Small arrows in panel j indicate large round-shaped macrophages infiltrating the adventitia. Statistical significance: p<0.05. (C) Percent area of Trail expression in medial and adventitial layers of ApoE-/-Opg+/+ (n = 16) and ApoE-/-Opg-/- (n = 16) mice. Measurements for all samples are presented as box plots. Measurements for the three groups are presented as bar graphs. n = 4 and 8 for the No Aneurysm group, n = 5 and 5 for the Aneurysm group, and n = 7 and 3 for the Dissection group in ApoE-/-Opg+/+ and ApoE-/-Opg-/- mice, respectively. Statistical significance: p<0.05.