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. 2020 Jul 2;15(7):e0235416. doi: 10.1371/journal.pone.0235416

Fig 3. GC-MS chromatograms (selected ion, m/z = 93) of PamTps1 products generated both in in vitro and in vivo systems.

Fig 3

PamTps1 enzymatic reaction incubated with (A) GPP or (B) FPP; products generated in E. coli harboring (C) empty vector and (D) PamTps1; (E) (-)-linalool standard and (F) cis- and trans-nerolidol standards. In vitro PamTps1 reactions produced exclusively linalool and nerolidol when incubated with GPP and FPP, respectively. In recombinant E. coli, PamTps1 produced both linalool and nerolidol. Only sample peaks higher than the negative control were marked with numbers. Corresponding compounds are: 1 = linalool (retention time = 6.7 min); 2 = trans-nerolidol (retention time = 13.0 min) and 3 = cis–nerolidol (retention time = 12.6 min).