Table 2. The forward and reverse sequences of primers for Phenylalanine Ammonia Lyase (McPAL; XM_022284778), 4-Coumaroyl CoA ligase (Mc4CL; XM_022281848, transcription Factor WRKY1 (NW_019104495), and Elongation factor (a housekeeping gene).
| Primer name | Sequence (5'-3') | Tm | Amplicon (bp) |
|---|---|---|---|
| PAL-F | ATTGGGAAGCTCATGTTTGC | 57 | 177 |
| PAL-R | GGTGACGGGATTTGCTAAGA | 57.6 | |
| WRKY1-F | AGTGATGGCATAGTCCTCGAT | 59 | 112 |
| WRKY1-R | CCAGGAATATGCGAACTTAGCTT | 59 | |
| 4CL-F | ACCTCTGTTCCATGTTTTCGGAT | 61 | 142 |
| 4CL-R | AGCAACCGATATGTACGTGACC | 61 | |
| EFa-F | GAACCGTGCTTGCTCAACCTC | 61 | 138 |
| EFa-R | AGCCGCAACAACTATGATCACC | 61 |