Table 1. Primers used in this work.
| Primer | Sequence | Enzyme |
|---|---|---|
| ADE3up-F | GATAAGCTTGATATCGAATTCCTGCAGCCCCCCGGGACGTAATGGAATAACTGCTGAC | SmaI |
| ADE3up-R | GAAGTTATGGATCCTACGAGGTAATTGAAGGCTCAC | |
| ADE3lox-F | CTTCAATTACCTCGTAGGATCCATAACTTCGTATAATG | |
| ADE3lox-R | CAATCTCTCCCTTGTCATCGGATCCATAACTTCGTATAG | |
| ADE3dw-F | GAAGTTATGGATCCGATGACAAGGGAGAGATTGAAG | |
| ADE3dw-R | GGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCCCCGGGTGCAATGTACTGTTGAGTAGG | SmaI |
| ADE3conF | GGGGACCGGAGGTAAAAGAC | |
| ADE3conR | GTTGGAATAATTGCATGGTCTG | |
| MUT1(Hpa) | CCATTGACATGGTAAACAGTTGGA | |
| MUT2(Bam) | GAACACTGGGATCTTGGTTGAGG | |
| Cen1-F | GAGTTTAAACGGATCCACGAAGCAATGGATAGGCACT | BamHI |
| Cen1-R | CCGCGAATTCGGATCCTGAAGTCTTTCAGAGAGGAGCA | BamHI |
| Cen1c-F | CAAGTATGCGTGATCCCAGGT | |
| Cen1c-R | TACGAATTGTGGGGCTCTGT | |
| Cen2-F | GAGTTTAAACGGATCCATCTCCGTTGATACTCCCAAC | BamHI |
| Cen2-R | CCGCGAATTCGGATCCATCGACAAGCAGAACACTAAG | BamHI |
| Cen2c-F | GAATGGAGGTGCTGGTGGTTA | |
| Cen2c-R | TGTAATGCTCGCTGGTGAGT | |
| Cen3-F | GAGTTTAAACGGATCCAAGTGGTACACCAGTCAGCG | BamHI |
| Cen3-R | CCGCGAATTCGGATCCTCAGTATTCAACTGCAACTGC | BamHI |
| Cen3c-F | TCAGCCGAATACCCACACTT | |
| Cen3c-R | TCAGCCGTCAGCGAAATGAT | |
| Cen4-F | GAGTTTAAACGGATCCCAAACGCACCGTCTTGTTCA | BamHI |
| Cen4-R | CCGCGAATTCGGATCCAATTGATGTAGACGAGCAGC | BamHI |
| Cen4c-F | TCAAGAATCGTACTGGCACCT | |
| Cen4c-R | CAAGCTCGTGAGATGGGATGT | |
| Cen370-F | CGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTGGTCATGAGATCAGATCTAACATCCAAAGACGAAAGGTTGAATGAGTTTAAACGGATCCAAGTGG | |
| Cen370-R | GCTGGCCCTCTCTTCCCAGCTCACGAATCAGATCCTAAGTCCTACTCAACAGTACATTGCAGCGGCCGCGTTTAAACGAATTCGGATCCTCAGTATTCAA | |
| qZEO-F | CGACGTGACCCTGTTCATCA | |
| qZEO-R | TGGACACGACCTCCGACCA | |
| qHIS-F | GTGTATCCTGGCTTGGCATCT | |
| qHIS-R | GCCAAGTACGGTGTGACGTT |
Restriction sites are underlined.