Fig 8. Expression of genes related to DNA methylation in Longissimus dorsi muscle of beef heifers that received rumen-protected methionine (RPM) for 45 days before transportation and heifers control (CTRL) that did not received RPM.

Statistically significant differences were declared at P < 0.05 and tendencies at P > 0.05 and < 0.1. Treatment × time interaction (***), time effect (**) and treatment effect (*). Tendencies are denoted if symbols (*, ** or ***) are underlined. Symbols (▲) on lines denote significant differences (P < 0.05) between two time points for the same treatment, symbols (●) denote significant differences (P < 0.05) between treatment at the same time point. Because the data presented in this chart are fold-change values, the baseline reference value is 1 for RPM and CTRL, therefore, there is overlap between symbols with error bars at baseline.