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. 2020 Jun 22;16(6):e1008892. doi: 10.1371/journal.pgen.1008892

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© 2020 Hong et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A—D) The expression pattern of the QS gene analyzed by promoter-GUS analysis. Histochemical staining of 10-day-old seedling (A), rosette leaf (B), flower (C) and silique (D) of the QSpro:QS-GUS transgenic plants. (E) The expression of QS in different tissues determined by qRT-PCR. Values are means ± SD (n = 3). (F, G) Subcellular localization of QS-YFP and qs-2-YFP fusion proteins. The YFP signals in the transgenic plants harboring the QSpro:QS-YFP (F) and QSpro:qs-2-YFP (G) were observed using confocal microscopy. YFP, yellow fluorescence protein. Chl, chlorophyll autofluorescence. BF, bright field. Bars, 10 μm. (H) Growth phenotypes of Col-0 and qs-2 mutant plants in soil for 35 days. Bars, 10 cm. (I—N) Analysis of the metabolites in the rosette leaves of Col-0 and qs-2 mutants. The contents of NAD (I), NaMN (J), NA (K), Tg (L), NAOG (M), and NANG (N) were determined in the rosette leaves of Col-0 and qs-2 plants grown in 1/2 MS for 3 weeks. The data represent the means ± SD (n = 5). Asterisks indicate significant differences from the Col-0 plants, *** P < 0.001, Student’s t-test.