(A) Gating strategy of ILCs in human pericardial fluid samples by CyTOF. L/D, live/dead.
(B) Representative CyTOF plots showing ILCs in the pericardial fluid from controls and patients with cardiac diseases. ARVC, arrhythmogenic right ventricular cardiomyopathy; IHD, ischemic heart disease.
(C) Frequency of ILCs in the pericardial fluid.
(D) Flow cytometry plots showing Lin−CD127+ ILCs in the pericardial fluid from controls and patients with pericarditis. Plots show a CD45+CD11b−CD3−CD19−CD56− population. Lin included CD1a, CD11c, CD14, CD34, CD123, CD303a(BDCA2), TCRαβ, and FcεRIα.
Concatenated samples (control, n = 3; pericarditis, n = 2) are shown in (D). One-way ANOVA followed by Tukey’s post hoc test (C) was used for statistical analysis. ***p < 0.001.