KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Table S1 | N/A | N/A |
| Biological Samples | ||
| Pericardial fluid samples | Johns Hopkins University School of Medicine | https://www.hopkinsmedicine.org/som/ |
| Pericardial fluid samples | Institute for Clinical and Experimental Medicine, Prague, Czech Republic | https://www.ikem.cz/en/ |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Mouse IL-33 (carrier-free) | BioLegend | Cat#580508 |
| Collagenase, Type 2 | Worthington | Cat#LS004177 |
| Deoxyribonuclease I | Worthington | Cat#LS002139 |
| Protease, Type XIV: Bacterial, From Streptomyces griseus | Sigma | Cat#P5147-5G |
| ACK Lysing Buffer | Quality Biological | Cat#118-156-721 |
| Bovine Serum Albumin | Sigma | Cat#A3608-100G |
| 0.5M EDTA, pH8.0 | Corning | Cat#46-034-CI |
| PMA | Sigma | Cat#P1585 |
| Ionomycin | Sigma | Cat#I9657 |
| BD GolgiPlug | BD Biosciences | Cat#555029 |
| BD GolgiStop | BD Biosciences | Cat#554724 |
| LIVE/DEAD Fixable Aqua Dead Cell Stain Kit | Thermo Fisher | Cat#L34966 |
| BV711 Streptavidin | BioLegend | Cat#405241 |
| Streptavidin Alexa Fluor 350 Conjugate | Thermo Fisher | Cat#S11249 |
| BD CytoFix Fixation Buffer | BD Biosciences | Cat#554655 |
| Fixation/Permeabilization Solution Set | BD Biosciences | Cat#554714 |
| eBioscience Foxp3/Transcription Factor Staining Buffer Set | Thermo Fisher | Cat#00-5523-00 |
| CountBright Absolute Counting Beads | Thermo Fisher | Cat#C36950 |
| SafeFix II All-Purpose Fixative | Fisher Scientific | Cat#23-042600 |
| DMEM | GIBCO | Cat#11995-065 |
| RPMI 1640 | GIBCO | Cat#11875-093 |
| Heat Inactivated Fetal Bovine Serum | Thermo Fisher | Cat#10082147 |
| L-Glutamine | Corning | Cat#25-005-CI |
| Penicillin-Streptomycin | Sigma | Cat#P4333 |
| Antibiotic-Antimycotic solution (100x) | Corning | Cat#30-004-CI |
| Sodium Pyruvate | Sigma | Cat#S8636 |
| 1M HEPES Buffer pH7.3 | Quality Biological | Cat#118-089-721 |
| MEM Non-essential Amino Acid solution (100x) | Sigma | Cat#M7145-100ML |
| 2-Mercaptoethanol | GIBCO | Cat#21985-023 |
| Recombinant Mouse IL-2 (carrier-free) | BioLegend | Cat#575404 |
| Recombinant Mouse IL-7 (carrier-free) | BioLegend | Cat#577802 |
| Heparin sodium salt from porcine intestinal mucosa | Sigma | Cat#H3393 |
| Trypsin 2.5% | Quality Biological | Cat#118-086-721 |
| Histopaque-1119 | Sigma | Cat#11191-100ML |
| Histopaque-1077 | Sigma | Cat#10771-100ML |
| CD90.2 MicroBeads, mouse | Miltenyi Biotec | Cat#130-049-101 |
| CD45R (B220) MicroBeads, mouse | Miltenyi Biotec | Cat#130-049-501 |
| TRIzol Reagent | Thermo Fisher | Cat#15596026 |
| iScript cDNA Synthesis Kit | Bio-Rad | Cat#1708891 |
| Power SYBR Green PCR Master Mix | Thermo Fisher | Cat#4367659 |
| Normal Goat Serum Blocking Solution | Vector Laboratories | Cat#S-1000-20 |
| Prolong Glass Antifade Mountant | Thermo Fisher | Cat#P36980 |
| Visikol HISTO Starter Kit | VISIKOL | Cat#HSK-1 |
| Forane Liquid for inhalation 100ml | Baxter | Cat#1001936040 |
| Buprenorphine(1mg/ml) | ZooPharm | Cat#BZ8069317 |
| Succinylcholine (20mg/ml) | Hospira, Inc. | Cat#00409662902 |
| CellTrace Violet Cell Proliferation Kit | Thermo Fisher | Cat#C34557 |
| CellTrace Far Red Cell Proliferation Kit | Thermo Fisher | Cat#C34564 |
| MyHCα614-629 (Ac-SLKLMATLFSTYASAD) | GenScript | N/A |
| Trilogy | Cell Marque | Cat#920P-04 |
| TBS with Tween (TBST), 20X Solution | Thermo Fisher | Cat#J75500-K2 |
| Dual Endogenous Enzyme Block | Dako | Cat#S2003 |
| Antibody Dilution Buffer | Ventana | Cat#ADB250 |
| Richard-Allan Scientific Signature Series Hematoxylin 1 | Thermo Fisher | Cat#7221 |
| Richard-Allan Scientific Signature Series Clear-Rite 3 | Thermo Fisher | Cat#6901 |
| Shandon Bluing Reagent | Thermo Fisher | Cat#6769001 |
| Optic Mount I Toluene | Mercedes Scientific | Cat#MER7722 |
| Maxpar PBS | Fluidigm | Cat#201058 |
| Cell-ID Intercalator-Ir | Fluidigm | Cat#201192B |
| Cell-ID Cisplatin | Fluidigm | Cat#201064 |
| Maxpar Cell Staining Buffer | Fluidigm | Cat#201068 |
| Maxpar Fix and Perm Buffer | Fluidigm | Cat#201067 |
| 16% Formaldehyde (w/v), Methanol-free | Thermo Fisher | Cat#28906 |
| Critical Commercial Assays | ||
| Mouse CCL11/Eotaxin-1 Quantikine ELISA Kit | R&D Systems | Cat#MME00 |
| Anti-Goat HRP-DAB Cell & Tissue Staining Kit | R&D Systems | Cat#CTS008 |
| Experimental Models: Organisms/Strains | ||
| Mouse: WT: BALB/cJ | The Jackson Laboratory | Cat#000651 |
| Mouse: CD45.1+: CByJ.SJL(B6)-Ptprca/J | The Jackson Laboratory | Cat#006584 |
| Mouse: Rag2−/−: C.B6(Cg)-Rag2tm1.1Cgn/J | The Jackson Laboratory | Cat#008448 |
| Mouse: Rag2−/−Il2rg−/−: C;129S4-Rag2tm1.1Flv Il2rgtm1.1Flv/J | The Jackson Laboratory | Cat#014593 |
| Mouse: ΔdblGATA1: C.129S1(B6)-Gata1tm6Sho/J | The Jackson Laboratory | Cat#005653 |
| Mouse: ST2−/− (BALB/c) | Townsend et al., 2000 (Dr. McKenzie) | N/A |
| Mouse: IL-13−/− (BALB/c) | McKenzie et al., 1998 (Dr. McKenzie) | N/A |
| Mouse: IL-33cit/+ (BALB/c) | Wong et al., 2012 (Dr. McKenzie) | N/A |
| Mouse: IL-33−/−: IL-33cit/cit (BALB/c) | This paper | N/A |
| Mouse: IL-5Tg (BALB/c) | Lee et al., 1997 (Dr. Lee) | N/A |
| Oligonucleotides | ||
| Ccl11 forward primer: 5′-GAATCACC AACAACAGATGCAC-3′ | This paper | N/A |
| Ccl11 reverse primer: 5′-ATCCTGGAC CCACTTCTTCTT-3’ | This paper | N/A |
| Ccl24 forward primer: 5′-TCTTAGG GCCCTTCTTGGTG-3’ | This paper | N/A |
| Ccl24 reverse primer: 5′-AATTCCAG AAAACCGAGTGG-3′ | This paper | N/A |
| Il5 forward primer: 5′-CTCTGTTG ACAAGCAATGAGACG-3′ | This paper | N/A |
| Il5 reverse primer: 5′-TCTTCAGT ATGTCTAGCCCCTG-3′ | This paper | N/A |
| Il13 forward primer: 5′-CCTGGCTCTTGCTTGCCTT-3′ | This paper | N/A |
| Il13 reverse primer: 5′-GGTCTTGT GTGATGTTGCTCA-3′ | This paper | N/A |
| Gapdh forward primer: 5′-TTGATGG CAACAATCTCCAC-3′ | This paper | N/A |
| Gapdh reverse primer: 5′-CGTCCC GTAGACAAAATGGT-3′ | This paper | N/A |
| Software and Algorithms | ||
| FlowJo | FlowJo | RRID: SCR_008520 |
| PRISM | GraphPad | RRID: SCR_002798 |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Imaris | BitPlane | RRID: SCR_007370 |
| Cytobank | Cytobank | RRID: SCR_014043 |