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. 2020;21(2):431–438. doi: 10.31557/APJCP.2020.21.2.431

Table 1.

The PCR Primers and Sequencing Primer Used for BRCA1 5382insc Founder Mutation Analysis by Pyrosequencing

Mutation Primer name Primer sequence* Product size(bp)
5382insC-F 5′-AAAGCGAGCAAGAGAATCCC-3′
BRCA1 5382insC 5382insC (biotinylated) 5′-TGGGGTGAGATTTTTGTCAAC-3′-biotin 72
Sequencing primer 5′-CGAGCAAGAGAATCCC-3′

*The GenBank references for primer sequences are: BRCA1 (NM 007304)