Table 1.
The PCR Primers and Sequencing Primer Used for BRCA1 5382insc Founder Mutation Analysis by Pyrosequencing
| Mutation | Primer name | Primer sequence* | Product size(bp) |
|---|---|---|---|
| 5382insC-F | 5′-AAAGCGAGCAAGAGAATCCC-3′ | ||
| BRCA1 5382insC | 5382insC (biotinylated) | 5′-TGGGGTGAGATTTTTGTCAAC-3′-biotin | 72 |
| Sequencing primer | 5′-CGAGCAAGAGAATCCC-3′ |
*The GenBank references for primer sequences are: BRCA1 (NM 007304)