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. 2020 Jun 8;9:e54276. doi: 10.7554/eLife.54276

Figure 2. The Bcd gradient noise of stau– mutants is comparable with that of the WT.

(A) The average intensity of the nuclear Bcd-GFP gradient of stau– mutants (blue) and the WT (black) as a function of the fractional embryo length at 16 min into nc14. Each circle and error bar represents the average and standard deviation, respectively, of the Bcd-GFP fluorescence intensity of the nuclei in the bin with a bin size of 2% EL. The inset shows the logarithm of the intensity as a function of the fractional embryo length and the linear fit in the range of 0.1–0.8 EL. (B) The relative Bcd-GFP gradient noise of stau– mutants (blue) and the WT (black). Each circle represents the standard deviation divided by the mean of the Bcd-GFP fluorescence intensity of the nuclei in the bin with a bin size of 2% EL. The error bar represents the standard deviation of the mean relative gradient noise calculated with bootstrap. (C) Variation in the average profiles of the Bcd gradient in stau– mutants from nc12 to 50 min into nc14. (D, E) The heat map of the Bcd gradient noise of the WT (D) and stau– mutants (E) from nc12 to 50 min into nc14. (F) The average Bcd gradient noise of stau– mutants from x = 0 to x = 0.55 EL versus developmental time is comparable with that of the WT.

Figure 2—source data 1. Bcd gradients measured at 16 min into nc14.
Source data for Figure 2A and B.

Figure 2.

Figure 2—figure supplement 1. Live imaging of dynamic Bcd gradients.

Figure 2—figure supplement 1.

(A) Comparison of the maximum projected image and the single image in the mid-sagittal plane from a z-stack of images of the fly embryos expressing Bcd-GFP in nc12. (B) The nuclear mask noise from imaging segmentation and the imaging shot noise is much smaller than the total Bcd-GFP gradient noise. (C) Comparison of the average nuclear fluorescence intensity of the control sample and the embryos measured in three independent sessions. (D) The average nuclear fluorescence intensity of the embryos is linearly correlated with that of the control sample in three measurement sessions. Blue circles represent data from each session, and the dash line represents the linear fitting.