Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (D. melanogaster) | w1118 | Yi Rao Lab | BDSC3605 | |
| Strain, strain background (D. melanogaster) | stauHL54 | This paper | See 'Materials and methods' | |
| Strain, strain background (D. melanogaster) | stauD3 | This paper | See 'Materials and methods' | |
| Strain, strain background (D. melanogaster) | bcd-egfp;+;bcdE1 | Thoms Gregor Lab | ||
| Strain, strain background (D. melanogaster) | bcd-egfp;stauHL54;bcdE1 | Thoms Gregor Lab | ||
| Antibody | Anti-Hb (mouse monoclonal) | Abcam | ab197787 | IF (1:1000) |
| Antibody | Anti-Kr (guinea pig polyclonal) | John Reinitz Lab | IF (1:1000) | |
| Antibody | Anti-Eve (rat polyclonal) | John Reinitz Lab | IF (1:1000) | |
| Antibody | Anti-guinea pig (goat monoclonal) | John Reinitz Lab | IF (1:1000) | |
| Antibody | Anti-rat (goat monoclonal) | Invitrogen | A11006 | IF (1:1000) |
| Antibody | Anti-mouse (goat monoclonal) | Invitrogen | A21240 | IF (1:1000) |
| Chemical compound, drug | Agarose gels | Invitrogen | E-Gel EX | 1~1.5% |
| Software, algorithm | 3D image processing | This paper | Source code files | See 'Materials and methods' |
| Software, algorithm | MATLAB | MATLAB, 2018 | MATLAB 2018a v9.4.0.813654 | |
| Other | DAPI stain | Invitrogen | D1306 | (1 µg/mL) |
| Other | Capillary | Brand | 701904 |