TABLE I.
Examples of reprogramming factors identified using high throughput screening.
| Starting cell | Ending cell | Screen type | Readout | Unique hit | References |
|---|---|---|---|---|---|
| Mouse epiblast stem cell | iPSC | CRISPRa | Oct4-GFP | Sall1 | 19 |
| Mouse embryonic fibroblast | iPSC | ORF overexpression | Nanog-GFP | Glis1 | 44 |
| Mouse embryonic fibroblast | iPSC | ORF overexpression | Tra-1–60+ | Hhex, Hlx | 45 |
| Mouse embryonic fibroblasts | iPSC | RNAi | Oct4-GFP | Trim28 | 46 |
| Mouse embryonic fibroblast | iPSC | RNAi | Alkaline phosphatase+ | Tox4 | 47 |
| Mouse embryonic fibroblast | iPSC | Chemical | Oct4-GFP | AM580+EPZ004777+SGC0946 + 5-aza-2-deoxycitidine | 48 |
| Human dermal fibroblast | iPSC | Chemical | Alkaline phosphatase+ | SAHA-PIP Ì | 49 |
| Mouse embryonic stem cell | Neuron | CRISPRa | Tubb3-CD8 | Ezh2 | 18 |
| Human fibroblasts | Neuron | ORF overexpression | scRNA-seq | Pax6+Neurog2+Dlx2+Zic1, Pax6+Neurog2 +Dlx1+Isl1 | 50 |
| Mouse embryonic fibroblasts | Neuron | ORF overexpression | Tau-GFP | Brn3c+Ascl1 | 51 |
| Mouse embryonic skin fibroblast | Neuron | Chemical | Tau-GFP | Forskolin+ISX9+ CHIR99021+I-BET151 | 52 |
| Human fetal lung fibroblasts | Neuron | Chemical | Map2+ | Kenpaullone+Prostaglandin E2+Forskolin+BML210+ Aminoresveratolsulfat+PP2 | 53 |
| Mouse cardiac fibroblast | Cardiac progenitor cell | CRISPR knockout | Nkx2-5-GFP | Dmap1 | 54 |
| Mouse cardiac fibroblasts | Cardiomyocytes | ORF overexpression | αMHC-GFP | Znf281 | 55 |
| Mouse cardiac fibroblasts | Cardiomyocytes | ORF overexpression | GCAMP+ | Hand2+Nkx2.5+Gata4 +Mef2c+Tbx5 | 56 |
| Mouse tail-tip fibroblasts | Cardiomyocytes | RNAi | αMHC-GFP | Bmi1 | 35 |
| Mouse cardiac fibroblast | Cardiomyocytes | RNAi | αMHC-GFP | Zrsr2 | 57 |
| Human cardiac fibroblasts | Cardiomyocytes | Chemical | Cardiac troponin T-GFP | SB431542+XAV939 | 58 |
| Human iPSC | Endothelial cell | ORF overexpression | Fitness+ scRNA-seq | Etv2 | 17 |
| Mouse embryonic fibroblast | Epicardial cell | ORF overexpression | scRNA-seq | Atf3+Gata6+Hand2 | 16 |
| Mouse embryonic fibroblasts | Dendritic cell | ORF overexpression | Clec9a-tdTomato | Pu.1+Irf8+Batf3 | 59 |
| Mouse embryonic stem cell | Primoridal germ cell | CRISPR knockout | Stella-GFP + Esg1-tdTomato | Nr5a2, Zfp296 | 60 |
| Mouse embryonic stem cell | 2C-like cell | CRISPRa | scRNA-seq | Dppa2, Smarca5 | 61 |