Figure 6.

Phylogenetic relationship of the helicase region of members of the family Flaviviridae. Partial gene sequences (∼280 aa) from the proposed helicase region were used for the phylogenetic analysis and included representative strains from each genus. CLUSTALX v1.81 was used to create a multiple alignment for the aa sequences which was verified by alignment of the known motifs in the region (e.g., GxGKS/T). An unrooted phylogenetic tree was constructed from the sequence alignment using the distance method, Neighbor-joining from the PAUP 4b10 package within a Macintosh environment. The virus names corresponding to the abbreviations can be found in the “List of Species” in each genus and the Genbank accession numbers are AF037405, U18059, M31182, J04358, M87512, M29095, U22303, U22304, NC 002348, AF144617, M62321, D17763, D00944, D10988, X61596, M18370, M12294, NC_002031. Bootstrap replicates of 1000 trees was also examined using PAUP and the percentages are drawn at the branch points of the tree.

(Courtesy of L. McMullan).