Extended Data Figure 1. Schematic of the Nativeomics workflow for identification of ligands from soluble and membrane proteins assemblies.

Successive rounds of MS/MS are applied to identify ligands bound to (a) soluble, and (b) membrane, protein assemblies. For multi-subunit complexes this can be achieved with or without subunit localisation of ligand binding, which if desired, adds at least one further MS/MS stage, as indicated in the flow schemes. (see Supplementary Table 1).