Skip to main content
. 2020 Jun 15;43(6):581–589. doi: 10.14348/molcells.2020.0032

Fig. 4. Signaling pathway of NELL2 action for axon development.

Fig. 4

(A) Expression of Robo1, Robo2, and Robo3 mRNA in the different developmental stage of hippocampal primary cells. (B) Robo2 and Robo3 mRNA levels were determined in the hippocampal primary cells transfected with negative control siRNA (siCTL), siRNA Robo2 (siRobo2), or siRNA Robo3 (siRobo3). (C and D) Quantitative analysis for the average neurite length (C) and axon length (D) by treatment with siRobo2 and siRobo3. n = 53 (siCTL), 56 (siRobo2), and 55 (siRobo3) cells. (E and F) Effect of ERK inhibitor (U0126, 10 µM) on NELL2 action on neurites and axon development: representative microphotographs showing primary cultured neurons (E) transfected with pDS-GFP-XB (CTL) or pDS-NELL2-GFP (NELL2) and calculated average neurite length (F). n = 46 (CTL), 28 (CTL-U0126), 43 (NELL2-CTL), and 22 (NELL2-U0126) cells. Scale bar = 20 µm. All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. AU, arbitrary units. P values for unpaired comparisons were analyzed by two-tailed Student’s t-test. Two-way repeated-measures ANOVA was performed to detect significant interaction between groups.