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. 2020 Jun 15;43(6):539–550. doi: 10.14348/molcells.2020.0098

Fig. 5. Comparison of expression of genes and proteins related to the interaction between GBM cells and brain-endothelial cells.

Fig. 5

(A) High expression of CXCR4 in LN229/GFP cells. The data is presented as mean ± SE from three independent experiments. *P < 0.05. (B) Representative western blot analysis showing the endogenous expression of STAT3 and p-STAT3 in U87 and LN229 cells. (C) Densitometry analysis of STAT3 and p-STAT3 immunoblots showing no significant difference between GBM cell lines. (D) The STAT3 and p-STAT3 immunoreactivity in the zones 2 and 3 of GBM-bearing tumors. Scale bar = 100 µm. (E) Double immunostaining for the presence of STAT3 (red) and GFP (green) showing co-localization (or proximity) of GFP and STAT3 immunoreactivity in the LN229/GFP-bearing tumors. Scale bar = 500 µm.