Skip to main content
. Author manuscript; available in PMC: 2021 Jul 6.
Published in final edited form as: Angew Chem Int Ed Engl. 2020 May 11;59(28):11312–11315. doi: 10.1002/anie.202002751

Figure 2.

Figure 2.

TET family enzymes are robust N-demethylases. (A) The F27–4mC substrate (200 nM) was reacted with or without TET2-CS (1.6 µM) and products were analyzed by ESI-MS with masses corresponding to substrates and potential products noted. A plausible mechanism for N-demethylation involving oxidation followed by deformylation is also shown. (B) The F27–4dmC substrate was reacted with TET2-CS or buffer and analyzed by ESI-MS. A plausible mechanism for di-demethylation involving iterative oxidation and deformylation is shown. (C) The F27–4dmC was reacted with TET1-FCD or TET3-FCD under conditions identical to the TET2-CS reaction and buffer only controls above and analyzed by ESI-MS.