REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-human IgG peroxidase	Sigma-Aldrich	Cat# A6029-1ML; RRID: AB_258272
CR3022	ter Meulen et al., 2006; Yuan et al., 2020	N/A
Goat anti-human IgG - H&L (HRP)	Abcam	Cat# ab6858-1MG; RRID: AB_955433
Phage display antibody set	Miersch et al., Bioraxiv https://doi.org/10.1101/2020.06.05.137349	N/A
VIR antibody set	Pinto et al., 2020	S309 PDB: 6WS6
Bacterial and Virus Strains
2019 n-CoV/USA_WA1/2020	CDC (gift from Natalie Thornburg)	N/A
Vaccinia virus vTF7-3	Fuerst et al., 1986	N/A
VSV-eGFP	Chandran et al., 2005	N/A
Biological Samples
Patient serum set	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
Blasticidin S HCl	GIBCO/Thermo Fisher	Cat# A1113903
Cytosine arabinoside	Sigma-Aldrich	Cat# C1768
Formaldehyde Solution	Millipore Sigma	Cat# FX0410-5
HEPES, free acid	Millipore Sigma	Cat #5310-OP
Hoechst 33342	Invitrogen/Thermo Fisher	Cat# H3570
Human ACE2-Fc	This study	GenBank: BAB40370.1 & AAC82527.1
L-[35S]-cysteine	Perkin Elmer	Cat# NEG022T
L-[35S]-methionine	Perkin Elmer	Cat# NEG009T
Lipofectamine 2000 Transfection Reagent	Invitrogen/Thermo Fisher	Cat# 11668019
Mouse ACE2-Fc	This study	NCBI Reference Sequence: NP_001123985.1
Phosphotungstic acid hydrate	Sigma-Aldrich	Cat# 79690-25G
Pierce ECL Western Blotting Substrate	Thermo Scientific/Thermo Fisher	Cat# 32106
Protein A Agarose Resin	GoldBio	Cat# P-400-100
TRIzol Reagent	Invitrogen/Thermo Fisher	Cat# 15596018
TrueBlue peroxidase substrate	KPL/SeraCare	Cat# 5510-0050
Critical Commercial Assays
Anti-SARS-CoV-2 ELISA (IgG) serology test	Euroimmun US, Inc., 1 Bloomfield Ave, Mountain Lakes, NJ 07046	N/A
Deposited Data
Nucleotide sequence of VSV-eGFP-SARS-CoV-2-SΔ21	This study	BioProject: PRJNA635934; SRA: SRR11878607
Experimental Models: Cell Lines
A549	Gift from Jan Carette	N/A
BHK-21	ATCC	Cat# CCL-10
BSRT7/5	Buchholz et al., 1999	N/A
Caco-2	ATCC	Cat# HTB-37
Caco-2 BBe1	ATCC	Cat# CRL-2102
Calu-3	Gift from Stanley Perlman	N/A
Expi293F	Thermo Fisher	Cat# A14527
ExpiCHO	GIBCO/Thermo Fisher	Cat# A29127
HEK293	ATCC	Cat# CRL-1573
HEK293T	ATCC	Cat# CRL-3216
H1Hela	Gift from Jan Carette	N/A
HepG2	ATCC	Cat# HB-8065
HT-29	ATCC	Cat# HTB-38
Huh7.5.1	Gift from Harry Greenberg
MA104	Gift from Harry Greenberg	N/A
Vero CCL81	ATCC	Cat# CCL-81
Vero E6	ATCC	Cat# CRL-1586
Vero E6-TMPRSS2	This paper	N/A
Vero-furin	Mukherjee et al., 2016	N/A
Experimental Models: Organisms/Strains
N/A	N/A	N/A
Oligonucleotides
TruSeq RNA Single Indexes Set A	Illumina	Cat# 20020492
TruSeq RNA Single Indexes Set B	Illumina	Cat# 20020493
Recombinant DNA
pCAGGS-VSV-G	Stanifer et al., 2011	N/A
pFM1.2-hACE2-Fc	This paper	GenBank: AB046569.1
pFM1.2-mACE2-Fc	This paper	NCBI Reference Sequence: NM_001130513.1
pGEM3-VSV L	Whelan et al., 1995	N/A
pGEM3-VSV G	Stanifer et al., 2011	N/A
pGEM3-VSV N	Whelan et al., 1995	N/A
pGEM3-VSV P	Whelan et al., 1995	N/A
pLX304-TMPRSS2	Zang et al., 2020	N/A
psPAX2	Gift from Didier Trono (unpublished data)	Addgene Cat# 12260
pVSV-eGFP	Chandran et al., 2005	N/A
pVSV-eGFP-SARS-CoV-2-SAA	This study	Spike mutated from MN908947.3
pVSV-eGFP-SARS-CoV-2-S MERS SAA cytoplasmic tail switch	This paper	Spike mutated from a codon-optimized version of AHI48572.1
pVSV-eGFP-SARS-CoV-2-S VSV G #1 cytoplasmic tail switch	This paper	VSV Indiana G (strain Orsay)
pVSV-eGFP-SARS-CoV-2-S VSV G #2 cytoplasmic tail switch	This paper	VSV Indiana G (strain Orsay)
pVSV-eGFP-SARS-CoV-2-S VSV G Ecto/TM/tail switch	This paper	VSV Indiana G (strain Orsay)
pVSV-eGFP-SARS-CoV-2-S VSV G TM/tail switch	This paper	VSV Indiana G (strain Orsay)
Software and Algorithms
BBMap 38.79	Bushnell et al., 2017	https://jgi.doe.gov/data-and-tools/bbtools/
Fastp 0.20.0	Chen et al., 2018	https://github.com/OpenGene/fastp
Image Analyses: GE InCell Analyzer 1000 Workstation	GE Life Sciences (now Cytiva)	N/A
NUCmer 3.1	Delcher et al., 2002	http://mummer.sourceforge.net/
SAMtools 1.9	Li et al., 2009	http://www.htslib.org/
SPAdes 3.13.0	Bankevich et al., 2012	http://cab.spbu.ru/software/spades/
Statistics: Prism 8.0	GraphPad	N/A
Other
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat	Illumina	Cat #20020596