Characterization of decellularized meniscus scaffolds. (a) dsDNA, (b) collagen, (c) GAGs levels of scaffolds were quantified by biochemical assay. (d) SEM images of mECM and Cowhide collagen scaffolds, Scale bar = 50 μm. (e) Cell viability of BMSCs mixed with mECM before and after extrusion from 25 G needle was assessed by confocal microscopy by live/dead staining (FDA, green; PI, red). Values are presented as means ± SD, n = 5. *, P < 0.05; relative to the normal group; Scale bar = 100 μm.