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. 2020 Jun 30;5(4):871–879. doi: 10.1016/j.bioactmat.2020.06.008

Fig. 3.

Fig. 3

In vitro fibrochondrogenesis of BMSCs in mECM versus Cowhide collagen. (a) Quantification of matrix production of GAG (n = 6) for cell proliferation. (b) qRT-PCR was used to analyze the gene expression levels of collagen I, collagen II and Aggrecan in vitro. (c) Cell skeleton staining of the cells cultured in the mECM or Cowihide collagen for 21 days (scale bar = 100 μm). (d) Alcian blue (top), Toluidine blue, Collagen II and Collagen I (bottom) staining of the cells cultured in the mECM or Cowihide collagen for 21 days (scale bar = 150 μm). Values are presented as means ± SD, n = 6. *, P < 0.05 relative to the normal group.