Figure 3.

Effect of N1-34a-NPs on TNBC Cell Viability, Proliferation, and Migration

(A) Representative images of SAβGal assay of MDA-MB-231 cells treated with IgG-Co-NPs, IgG-34a-NPs, N1-Co-NPs, or N1-34a-NPs. Green color indicates β-galactosidase activity. Red arrows indicate cells with disperse staining. Scale bars= 50 μm. (B) Percent proliferation of MDA-MB-231 cells treated with IgG-Co-NPs, IgG-34a-NPs, N1-Co-NPs, or N1-34a-NPs (n = 4). Error bars indicate standard error. ∗p < 0.05. (C) Normalized number of GFP-expressing MDA-MB-231 cells migrated through the transwell insert after treatment with IgG-Co-NPs, IgG-34a-NPs, N1-Co-NPs, or N1-34a-NPs (n = 3). (D) Representative fluorescence images of GFP-expressing MDA-MB-231 cells migrated through the transwell insert after treatment with IgG-Co-NPs, IgG-34a-NPs, N1-Co-NPs, or N1-34a-NPs. See also Figure S2. Scale bars = 500 μm