Table 2.
Virus namea | Original host strain | Contig No. | Total read count | Genus | Segment lengthb | Blastp | Protein size | Symptomc | |||
---|---|---|---|---|---|---|---|---|---|---|---|
dsRNA1/(accession) | Hit with highest score in | Identity | RdRp (aa) | R.n. | C.p. | ||||||
dsRNA2 (accession) | Blastp | (%) | Cp (aa) | Δdcl2 | EP155 | ||||||
RnPV11 | W98 | 4 262 |
61129 26713 |
Beta- | 2445 (LC517370) 2326 (LC517371) |
Rosellinia necatrix partitivirus 20 Fusarium poae partitivirus 2 |
71 35 |
757 652 |
–~+ | ++ | +++ |
RnPV12 | W118 | 148 779 |
47804 1197 |
Alpha- |
1845 LC517372
1925 (LC517373) |
Botrytis cinerea partitivirus 2 Erysiphe necator partitivirus 1 |
63 38 |
551 533 |
–~+ | ||
RnPV13 | W118 | 610 529 |
6211 4558 |
Alpha- | 1965 (LC517374)*
1822 (LC517375) |
Rhizoctonia oryzae-sativae partitivirus 1 Rhizoctonia oryzae-sativae partitivirus 1 |
59 54 |
469 488 |
|||
RnPV3 f | W118 | 317 347 |
Beta- | 2246 (LC010950) 2065 (LC010951) |
Rosellinia necatrix partitivirus 3 Rosellinia necatrix partitivirus 3 |
99 96 |
709 613 |
– | – | ||
RnPV14 | W744 | 118 750 |
46844 2477 |
Beta- |
2412 (LC517376)
2427 (LC517377) |
Rosellinia necatrix partitivirus 4 Rosellinia necatrix partitivirus 1-W8 |
72 47 |
743 706 |
–~+ | ++ | |
RnPV15 | W744 | 826 2267 |
7917 3491 |
Beta- | 2517 (LC517378) 2358 (LC517379) |
Rosellinia necatrix partitivirus 14 Rosellinia necatrix partitivirus 16 |
59 49 |
747 663 |
|||
RnPV16 | W744 | 509 3216 |
20228 944 |
Beta- | 2372 (LC517380) 2344 (LC517381) |
Podosphaera prunicola partitivirus 4 Rosellinia necatrix partitivirus 15 |
61 49 |
730 662 |
++ | +++ | |
RnPV17 | W744 | 671 237 |
4185 8985 |
Beta- | 2292 (LC517382) 2235 (LC517383) |
Heterobasidion partitivirus 7 Rhizoctonia solani partitivirus 7 |
63 71 |
725 670 |
|||
RnPV1f | W744/ W1134 |
43 82/69 |
Beta- | 2374 (AB113347) 2263 (AB113348) |
Rosellinia necatrix partitivirus 1-W8 Rosellinia necatrix partitivirus 1-W8 |
98 99/94 |
709 686 |
||||
RnPV18d | W442 | 110 874 |
30695 | Beta- | 2410 (LC517384) 2337 (LC517385) |
Enteoleuca partitivirus 2 Dill cryptic virus 2 |
99.7 43 |
740 670 |
– | – | – |
RnPV19d | W442 | 26 379 |
40899 5244 |
Alpha- | 2013 (LC517386) 1842 (LC517387) |
Entoleuca partitivirus 1 Grosmannia clavigera partitivirus 1 |
99.7 42 |
606 520 |
+ | – | |
RnPV20 | W1134 | 5 7 |
186156 55670 |
Beta- | 2417 (LC517388) 2318 (LC517389) |
Rosellinia necatrix partitivirus 11 Ceratobasidium partitivirus CP-h |
71 61 |
755 643 |
–~+ | +++ | +++ |
RnPV21 | W1134 | 161 69 |
49075 3784 |
Beta- |
2352 (LC517390)
2361 (LC517391) |
Podosphaera prunicola partitivirus 4 Rosellinia necatrix partitivirus 1-W8 |
65 94 |
721 686 |
|||
RnPV22 | W1050/ W1126 |
27 707 |
Alpha- |
2012 (LC517392)
2037 (LC517393) |
Trichoderma atroviride partitivirus 1 Trichoderma atroviride partitivirus 1 |
76 43 |
613 584 |
||||
RnPV23 | W662 | 127 25 |
Alpha- | 1831 (LC517394)**
1791 (LC517395)** |
Heterobasidion partitivirus 13 Heterobasidion partitivirus 13 |
49 37 |
571 514 |
||||
RnPV24 | W662 | 77 125 |
Alpha- | 1946 (LC517396)**
1771 (LC517397)** |
Oyster mushroom isometric virus II Medicago sativa alphapartitivirus 1 |
75 28 |
598 498 |
||||
RnPV25 | W129 | 51 188 |
Beta- | 2374 (LC517398)**
2049 (LC517399)** |
Trichoderma citrinoviride partitivirus 1 Trichoderma citrinoviride partitivirus 1 |
65 54 |
733 642 |
||||
RnPV10 | Rn459 | Alpha- | 1896 (LC333736) 1911 (LC333737) |
Rosellinia necatrix partitivirus 10 Rosellinia necatrix partitivirus 10 |
100 100 |
573 539 |
|||||
RnPV4e | W1030/ W1031 |
4 270 |
Alpha- | 2342 (AB698493) 2295 (LC521312) |
Rosellinia necatrix partitivirus 4 Sclerotinia sclerotiorum partitivirus 1 |
100 48 |
744 683 |
||||
RnPV5e | W1040/ W1041 |
10/522 27/62 |
Beta- | 2046 (AB698494) 1906 (LC521313) |
Rosellinia necatrix partitivirus 5 Trichoderma atroviride partitivirus 1 |
99.7 44 |
647 576 |
||||
RnPV6f | W558 | 52 137 |
Beta- | 2499 (LC010952) 2462 (LC010953) |
Rosellinia necatrix partitivirus 6 Rosellinia necatrix partitivirus 6 |
100 100 |
756 729 |
–~+ | +++ | +++ |
RnPV22 was also detected from the W1126 strain.
5′-terminal sequence of dsRNA1 is incomplete;
5′- and 3′-terminal sequences of both segments are incomplete. Underlined: RdRp is encoded by the smaller dsRNA segments.
R.n., R. necatrix W97 strain; C.p., C. parasitica EP155 or Δdcl2 strain. Growth reduction: –, No; +: Slight; ++, Moderate; +++, Great. See Figure 4 and Supplementary Figure S4 for colony morphology of virus-infected fungal strains.
Near-complete or complete dsRNA1 sequences of these viruses from Spanish Entoleuca sp. (d) or R. necatrix (e) have already been deposited in GenBank.
Partitiviruses belonging to the same species have previously been reported from different Japanese strains of R. necatrix.