Table 2.
Properties of newly discovered partitiviruses from Rosellinia necatrix.
| Virus namea | Original host strain | Contig No. | Total read count | Genus | Segment lengthb | Blastp | Protein size | Symptomc | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| dsRNA1/(accession) | Hit with highest score in | Identity | RdRp (aa) | R.n. | C.p. | ||||||
| dsRNA2 (accession) | Blastp | (%) | Cp (aa) | Δdcl2 | EP155 | ||||||
| RnPV11 | W98 | 4 262 |
61129 26713 |
Beta- | 2445 (LC517370) 2326 (LC517371) |
Rosellinia necatrix partitivirus 20 Fusarium poae partitivirus 2 |
71 35 |
757 652 |
–~+ | ++ | +++ |
| RnPV12 | W118 | 148 779 |
47804 1197 |
Alpha- |
1845 LC517372
1925 (LC517373) |
Botrytis cinerea partitivirus 2 Erysiphe necator partitivirus 1 |
63 38 |
551 533 |
–~+ | ||
| RnPV13 | W118 | 610 529 |
6211 4558 |
Alpha- | 1965 (LC517374)*
1822 (LC517375) |
Rhizoctonia oryzae-sativae partitivirus 1 Rhizoctonia oryzae-sativae partitivirus 1 |
59 54 |
469 488 |
|||
| RnPV3 f | W118 | 317 347 |
Beta- | 2246 (LC010950) 2065 (LC010951) |
Rosellinia necatrix partitivirus 3 Rosellinia necatrix partitivirus 3 |
99 96 |
709 613 |
– | – | ||
| RnPV14 | W744 | 118 750 |
46844 2477 |
Beta- |
2412 (LC517376)
2427 (LC517377) |
Rosellinia necatrix partitivirus 4 Rosellinia necatrix partitivirus 1-W8 |
72 47 |
743 706 |
–~+ | ++ | |
| RnPV15 | W744 | 826 2267 |
7917 3491 |
Beta- | 2517 (LC517378) 2358 (LC517379) |
Rosellinia necatrix partitivirus 14 Rosellinia necatrix partitivirus 16 |
59 49 |
747 663 |
|||
| RnPV16 | W744 | 509 3216 |
20228 944 |
Beta- | 2372 (LC517380) 2344 (LC517381) |
Podosphaera prunicola partitivirus 4 Rosellinia necatrix partitivirus 15 |
61 49 |
730 662 |
++ | +++ | |
| RnPV17 | W744 | 671 237 |
4185 8985 |
Beta- | 2292 (LC517382) 2235 (LC517383) |
Heterobasidion partitivirus 7 Rhizoctonia solani partitivirus 7 |
63 71 |
725 670 |
|||
| RnPV1f | W744/ W1134 |
43 82/69 |
Beta- | 2374 (AB113347) 2263 (AB113348) |
Rosellinia necatrix partitivirus 1-W8 Rosellinia necatrix partitivirus 1-W8 |
98 99/94 |
709 686 |
||||
| RnPV18d | W442 | 110 874 |
30695 | Beta- | 2410 (LC517384) 2337 (LC517385) |
Enteoleuca partitivirus 2 Dill cryptic virus 2 |
99.7 43 |
740 670 |
– | – | – |
| RnPV19d | W442 | 26 379 |
40899 5244 |
Alpha- | 2013 (LC517386) 1842 (LC517387) |
Entoleuca partitivirus 1 Grosmannia clavigera partitivirus 1 |
99.7 42 |
606 520 |
+ | – | |
| RnPV20 | W1134 | 5 7 |
186156 55670 |
Beta- | 2417 (LC517388) 2318 (LC517389) |
Rosellinia necatrix partitivirus 11 Ceratobasidium partitivirus CP-h |
71 61 |
755 643 |
–~+ | +++ | +++ |
| RnPV21 | W1134 | 161 69 |
49075 3784 |
Beta- |
2352 (LC517390)
2361 (LC517391) |
Podosphaera prunicola partitivirus 4 Rosellinia necatrix partitivirus 1-W8 |
65 94 |
721 686 |
|||
| RnPV22 | W1050/ W1126 |
27 707 |
Alpha- |
2012 (LC517392)
2037 (LC517393) |
Trichoderma atroviride partitivirus 1 Trichoderma atroviride partitivirus 1 |
76 43 |
613 584 |
||||
| RnPV23 | W662 | 127 25 |
Alpha- | 1831 (LC517394)**
1791 (LC517395)** |
Heterobasidion partitivirus 13 Heterobasidion partitivirus 13 |
49 37 |
571 514 |
||||
| RnPV24 | W662 | 77 125 |
Alpha- | 1946 (LC517396)**
1771 (LC517397)** |
Oyster mushroom isometric virus II Medicago sativa alphapartitivirus 1 |
75 28 |
598 498 |
||||
| RnPV25 | W129 | 51 188 |
Beta- | 2374 (LC517398)**
2049 (LC517399)** |
Trichoderma citrinoviride partitivirus 1 Trichoderma citrinoviride partitivirus 1 |
65 54 |
733 642 |
||||
| RnPV10 | Rn459 | Alpha- | 1896 (LC333736) 1911 (LC333737) |
Rosellinia necatrix partitivirus 10 Rosellinia necatrix partitivirus 10 |
100 100 |
573 539 |
|||||
| RnPV4e | W1030/ W1031 |
4 270 |
Alpha- | 2342 (AB698493) 2295 (LC521312) |
Rosellinia necatrix partitivirus 4 Sclerotinia sclerotiorum partitivirus 1 |
100 48 |
744 683 |
||||
| RnPV5e | W1040/ W1041 |
10/522 27/62 |
Beta- | 2046 (AB698494) 1906 (LC521313) |
Rosellinia necatrix partitivirus 5 Trichoderma atroviride partitivirus 1 |
99.7 44 |
647 576 |
||||
| RnPV6f | W558 | 52 137 |
Beta- | 2499 (LC010952) 2462 (LC010953) |
Rosellinia necatrix partitivirus 6 Rosellinia necatrix partitivirus 6 |
100 100 |
756 729 |
–~+ | +++ | +++ | |
a
RnPV22 was also detected from the W1126 strain.
b*
5′-terminal sequence of dsRNA1 is incomplete;
**
5′- and 3′-terminal sequences of both segments are incomplete. Underlined: RdRp is encoded by the smaller dsRNA segments.
c
R.n., R. necatrix W97 strain; C.p., C. parasitica EP155 or Δdcl2 strain. Growth reduction: –, No; +: Slight; ++, Moderate; +++, Great. See Figure 4 and Supplementary Figure S4 for colony morphology of virus-infected fungal strains.
d,e
Near-complete or complete dsRNA1 sequences of these viruses from Spanish Entoleuca sp. (d) or R. necatrix (e) have already been deposited in GenBank.
f
Partitiviruses belonging to the same species have previously been reported from different Japanese strains of R. necatrix.