Figure 4.

As₂O₃ impairs pDCs' capacity to induce CD4⁺ T cell proliferation and Th1/Th22 polarization. A pDC/CD4⁺ cell co-culture system was used. T cell proliferation and polarization was detected on day-5 and day-7 of co-culture, respectively. (A) Representative graph of cell proliferation (n = 4). (B) CD4⁺ T cells negative for cell proliferation dye (n = 4). (C) Representative graph of IFN-γ⁺ and TNF-α⁺ proliferating T cells gated on cell proliferation dye-negative cells (n = 4). (D) Percent of IFN-γ⁺ proliferating T cells (n = 4). (E) Representative graph of IFN-γ⁺ and IL-22⁺ proliferating T cells gated on cell proliferation dye-negative cells (n = 4). (F) % of IL-22⁺ proliferating T cells (n = 4). Data are represented as mean ± SEM. *P < 0.05, ^**P < 0.01 by t-test.