Figure 5.

As₂O₃ reduces pDCs' ability to induce plasmablast differentiation of B cells. A pDC/B cell co-culture model was used. CD38^hiCD27^hi plasmablast differentiation was analyzed on day-3 of co-culture. (A) Representative graph of CD38^hiCD27^hi plasmablasts, gated on CD19⁺ cells (n = 4). (B) Percent of CD38^hiCD27^hi plasmablasts among all gated B cells (n = 4). For (C) to (D), isolated pDCs were incubated with/without CpG-P activation, with indicated doses of As₂O₃ for 24 h before analysis. (C) IFN-α and IL-6 concentrations in supernatants of purified pDCs (n = 4 HD). (D) RFI ratios and percentages of positive cells for CD86 and PD-L1 (n = 4 HD). Data are represented as mean ± SEM. *P < 0.05, ^**P < 0.01 by t-test.