Figure 6.

As₂O₃-induced pro-apoptotic effects, selective IFN-α inhibition, and regulatory phenotype are not resistant by SSc pDCs. (A) Percent of IFN-α and TNF-α positive pDCs after PBMCs from SSc patients were incubated with indicated doses of As₂O₃ for 6 h [n = 12 SSc patients (SP)]. For (B) to (G), purified SSc pDCs were cultured with indicated doses of As₂O₃ before tests. (B) Percent of viable SSc pDCs after 24 h (n = 5 SP). (C) Percent of BAX⁺ cells, and MFI of BAX expression in all pDCs, after 6 h (n = 3 SP). (D) MFI of BCL-2 in all pDCs after 6 h (n = 3 SP). (E) BAX/BCL-2 MFI ratio after 6 h (n = 3 SP). (F) RFI ratios and (G) Percent of positive cells for indicated surface molecules, after 24 h (n = 5 SP). Data are represented as mean ± SEM. *P < 0.05, ^**P < 0.01 and ^***P < 0.001 by t-test.