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. 2020 Jul 2;40(7):BSR20192313. doi: 10.1042/BSR20192313

Table 1. PCR conditions and enzymes.

SNPs Primer sequences PCR conditions PCR products Digestion products Enzymes
NOD1/CARD4 (rs5743336) F: 5′-TGAGACCATCTTCATCCTGG-3′
R: 5′-CTTCCCACTGAGCAGGTTG-3′
94°C, 30 s
94°C, 30 s
56,8°C, 60 s
68°C, 60 s
68°C, 5 min
379 bp AA: 379 bp
AG: 379 bp, 209 bp, 170 bp
GG: 209 bp, 170 bp
Ava I 37°C
1 h
NOD2/CARD15 (rs2066847) F: 5′-GGCAGAAGCCCTCCTGCAGGGCC-3′
R: 5′-CCTCAAAATTCTGCCATTCC-3′
94°C, 30 s
94°C, 30 s
55,1°C, 60 s
68°C, 60 s
68°C, 5 min
151 bp WT/WT: 151 bp
WT/insC: 151 bp, 130 bp, 21 bp
insC/insC: 130 bp, 21 bp
Apa I 37°C
1 h