Table 1. PCR conditions and enzymes.
SNPs | Primer sequences | PCR conditions | PCR products | Digestion products | Enzymes |
---|---|---|---|---|---|
NOD1/CARD4 (rs5743336) | F: 5′-TGAGACCATCTTCATCCTGG-3′ R: 5′-CTTCCCACTGAGCAGGTTG-3′ |
94°C, 30 s 94°C, 30 s 56,8°C, 60 s 68°C, 60 s 68°C, 5 min |
379 bp | AA: 379 bp AG: 379 bp, 209 bp, 170 bp GG: 209 bp, 170 bp |
Ava I 37°C 1 h |
NOD2/CARD15 (rs2066847) | F: 5′-GGCAGAAGCCCTCCTGCAGGGCC-3′ R: 5′-CCTCAAAATTCTGCCATTCC-3′ |
94°C, 30 s 94°C, 30 s 55,1°C, 60 s 68°C, 60 s 68°C, 5 min |
151 bp | WT/WT: 151 bp WT/insC: 151 bp, 130 bp, 21 bp insC/insC: 130 bp, 21 bp |
Apa I 37°C 1 h |