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. 2020 Jun 26;14:538. doi: 10.3389/fnins.2020.00538

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Copyright © 2020 Eura, Matsui, Luginbühl, Matsubayashi, Nanaura, Shiota, Kinugawa, Iguchi, Kiriyama, Zheng, Kouno, Lan, Kongpracha, Wiriyasermkul, Sakaguchi, Nagata, Komeda, Morikawa, Kitayoshi, Jong, Kobashigawa, Nakanishi, Hasegawa, Saito, Shiromizu, Nishimura, Kasai, Takeda, Kobayashi, Inagaki, Tanaka, Makinodan, Kishimoto, Kuniyasu, Nagamori, Muotri, Shin, Sugie and Mori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative PCR and immunohistochemical analysis of 1-month-old hBSOs from hESCs. (A) Quantitative PCR analysis of 1-month-old hBSOs for the markers of neural stem/progenitor cell (SOX2, Mash1, SLC1A3), mature neuron (MAP2), midbrain (OTX2), and mDA (FOXA2, NR4A2, TH). Error bars indicate mean ± SEM; *p = 0.0167 (ASCL1), *p = 0.0412 (OTX2), *p = 0.0387 (NR4A2), ****p < 0.0001 (MAP2), *p = 0.0178 (TH). (B) Immunohistochemical staining of midbrain marker (OTX2), mDA marker (TH), and neural stem cell marker (SOX2) at 1 month. Bars = 100 μm.