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A, B
Recording of GFP‐expressing control (A) and NachBac‐expressing PVH CRH neurons (B) with a step‐wise depolarization protocol to assess least depolarization required to reach threshold for action potential firing (i.e., rheobase).
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C
Levels of corticosterone measured from blood obtained at the indicated time points in control and NachBac mice (12 weeks old, males, n = 6–7 each, data presented as mean ± SEM, *P = 0.051 control versus NachBac at time 22:00, two‐way ANOVA test).
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D
CRH immunostaining in the median eminence (red, middle) in CRH‐Cre mice injected with AAV‐Flex‐GFP (top panels) or NachBac vectors (bottom panels) to bilateral PVH.
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E
CRH‐Cre mice injected with control AAV‐Flex‐GFP vectors (GFP) or NachBac vectors (NachBac) to bilateral PVH, and CRH immunostaining in mice perfused at morning (9–10 am) or night (7–8 pm), and brain sections were obtained for CRH immunostaining (red). In both NachBac groups, a number of neurons exhibited CRH‐immunoreactive structures (arrows) whereas none was observed in either of GFP groups.
Data information: Scale bar = 200 μm.
