Figure 3. Clamping PVH CRH neuron activity at high levels causes rapid diet‐induced obesity.

• A–I
  CRH‐Cre male mice received stereotaxic delivery of AAV‐EF1a‐Flex‐EGFP‐P2A‐mNachBac or control GFP vectors at 7–8 weeks of age and were maintained on chow diet for 7 weeks before switching to HFD. Weekly body weight gain after viral delivery (A), and measurements of fat mass (B) and lean mass (C) at 11 weeks after viral delivery. (D–F) Measurements of energy expenditure during the 7^th week after viral delivery. Comparison of real‐time O₂ consumption traces during the 4‐day measurement period (D), of average O₂ consumption between the two groups (E), and of the difference between day and night periods between the two groups (F). (G–I) Measurements of feeding during the 7^th week after viral delivery. Comparison of real‐time feeding c traces during the 4‐day measurement period (G), of average food intake between the two groups (H), and of the difference in food intake between day and night periods between the two groups (I). n = 5–6 each, *P < 0.0001 in A, *P = 0.0019 in B, P = 0.2316 in C, *P = 0.0196 in E, *P = 0.0466 in F, *P = 0.0062 in H, and *P = 0.0298 in I, unpaired Student's t‐tests. All data presented as mean ± SEM.

Source data are available online for this figure.