Figure 5. Clamping PVH CRH neuron activity at low levels causes diet‐induced obesity.

• A–I
  CRH‐Cre male mice received stereotaxic delivery of AAV‐EF1a‐DIO‐Kir2.1‐P2A‐dTomato or control AAV‐Flex‐mCherry vectors at 7–8 weeks of age and were maintained on chow diet for 7 weeks before switching to HFD. Weekly body weight gain after viral delivery (A, *P < 0.0001, unpaired Student's t‐test), and measurements of fat mass (B, **P = 0.0006, unpaired Student's t‐test) and lean mass (C, P = 0.3728, unpaired Student's t‐test) at 15 weeks after viral delivery. (D–F) Measurements of energy expenditure during the 7^th week after viral delivery. Comparison of real‐time O₂ consumption traces during the 4‐day measurement period (D), of average O₂ consumption between the two groups (E, *P = 0.0007, unpaired Student's t‐test), and of the difference between day and night periods between the two groups (F, P = 0.0791, unpaired Student's t‐test). (G–I) Measurements of feeding during the 7^th week after viral delivery. Comparison of real‐time feeding c traces during the 4‐day measurement period (G), of average food intake between the two groups (H, **P = 0.0019, unpaired Student's t‐test), and of the difference in food intake between day and night periods between the two groups (I, *P = 0.0363, unpaired Student's t‐test). n = 6 each. All data presented as mean ± SEM.

Source data are available online for this figure.