Figure 1. Perinatal lethality of CARD14E138A transgenic mice is rescued by MALT1 deficiency.

1. Schematic representation of the Rosa26^{LSL‐CARD14‐E138A} transgene construct (LSL = LoxP‐stop‐LoxP).
2. Ratio of genotypes obtained of 1‐week‐old pups after crossing K5cre^Tg/+ CARD14E138A^+/+ male mice with K5cre^+/+ CARD14E138A^Tg/+ female mice; CARD14E138A^+/+ = Rosa26^+/+, CARD14E138A^Tg/+ = Rosa26^{LSL‐CARD14‐E138A/+}
3. Representative Western blot showing CARD14 protein levels in skin and liver lysates of E18.5 pups. Actin is shown as a loading control.
4. Representative images of K5cre^Tg/+ CARD14E138A^+/+ and K5cre^Tg/+ CARD14E138A^Tg/+ pups at E18.5.
5. Ratio of genotypes obtained of 1‐week‐old pups from crossing Malt1 ^fl/fl K5cre^Tg/+ CARD1414E138A^+/+ male mice with Malt1 ^fl/fl K5cre^+/+ CARD14E138A^Tg/+ female mice.
6. Representative image of 6‐month‐old Malt1 ^fl/fl K5cre^Tg/+ CARD14E138A^+/+ and Malt1 ^fl/fl K5cre^Tg/+ CARD14E138A^Tg/+ mice.
7. Body weight and ear thickness of untreated WT and Malt1 ^fl/fl K5cre^Tg/+ CARD14E138A^Tg/+ male and female mice (between 4 and 7 months old). Each symbol represents one mouse; the line represents the mean value (n ≥ 4 mice per group). WT = Malt1 ^fl/fl K5cre^+/+ CARD14E138A^+/+, Malt1 ^fl/fl K5cre^+/+ CARD14E138A^Tg/+, and Malt1 ^fl/fl K5cre^Tg/+ CARD14E138A^+/+ mice. Statistical difference between two groups was determined using a Mann–Whitney U‐test (ns: P > 0.05)
8. Representative H&E‐stained histological sections of ear and skin tissue of Malt1 ^fl/fl K5cre^+/+ CARD14E138A^+/+ and Malt1 ^fl/fl K5cre^Tg/+ CARD14E138A^Tg/+ mice (scale bar represents 100 μm).

Source data are available online for this figure.