Figure 2. Keratinocyte‐specific deletion of MALT1 reduces CARD14E138A‐induced psoriasiform inflammation.

1. Schematic representation of the experimental induction of CARD14^E138A transgene using tamoxifen. WT = K14creER^Tg/+ Rosa26^+/+, ieCARD14^E138A = K14creER^Tg/+ Rosa26^{LSL‐CARD14‐E138A/+}
2. Changes in relative body weight and ear thickness upon CARD14^E138A induction with tamoxifen. Malt1 ^EKO = Malt1 ^fl/fl. The combined results of five independent experiments are shown (Malt1 ^+/+ WT n = 17, Malt1 ^fl/fl WT n = 8, Malt1 ^+/+ ieCARD14^E138A n = 18, Malt1 ^fl/fl ieCARD14^E138A n = 12).
3. Representative H&E‐stained histological sections of ear tissue of tamoxifen‐treated mice (scale bar represents 200 μm). Arrowheads indicate infiltrates of immune cells.
4. Epidermal thickness measured on ear sections. Each symbol represents the mean of at least ten measurements for each ear; the line represents the mean value. The combined results of three independent experiments are shown.
5. Analysis of infiltrating immune cells in the ears of tamoxifen‐treated mice using flow cytometry. Cell count of neutrophils (CD45⁺ CD3/CD19⁻ CD64⁻ CD11b⁺ Ly6G⁺), eosinophils (CD45⁺ CD3/CD19⁻ CD64⁻ Ly6G⁻ SiglecF⁺), T cells (CD45⁺ CD3/CD19⁺, MHCII⁻), and DCs (CD45⁺ CD3/CD19⁻ CD64⁻ MHCII⁺CD11c⁺) in single cell suspensions of the ear (n ≥ 4).
6. mRNA expression levels of the indicated genes in ears relative to reference genes (Hprt1, Rpl13a, and Tbp1). Each symbol represents one mouse; the line represents the mean value (n ≥ 3).

Data information: Statistics: Error bars reflect SEM. Differences between Malt1 ^+/+ ieCARD14^E138A and Malt1 ^EKO ieCARD14^E138A groups were determined using REML analysis (B) or a Mann–Whitney U‐test (D–F) (*P < 0.05, **P < 0.01, ****P < 0.0001).