Figure 6.

MiR-142-3p upregulation enhanced drug sensitivity of breast cancer cells through suppressing autophagy by targeting HMGB1. Transfected MCF-7 (A) and MCF-7/DOX cells (B) were treated with different doses of DOX (0.1625, 0.3125, 0.625, 1.25, 2.5 and 5 μmol/L) for 48 h, respectively, then MTT assay was used to determine the cell viability and IC₅₀ value. Transfected MCF-7 (C) and MCF-7/DOX cells (D) were treated with 0.15 or 3 μmol/L DOX for 48 h, respectively, followed by the detection of apoptotic rate by flow cytometry. (E) The levels of ATG5, LC3-I and LC3-II in MCF-7 cells transfected with anti-miR-NC, anti-miR-142-3p, or anti-miR-142-3p + si-HMGB1 were detected by Western blot. (F) The levels of ATG5, LC3-I, and LC3-II in MCF-7/DOX cells transfected with miR-NC, miR-142-3p, or miR-142-3p + pcDNA-HMGB1 were detected by Western blot. Columns show the mean values of three experiments (±SD). *P < 0.05.