Skip to main content
. 2020 Jun 26;11:951. doi: 10.3389/fphar.2020.00951

Figure 3.

Figure 3

Sit induces autophagy in the perforator flap. (A) Autophagosomes (red) in cells in SCZ of flaps in the control, Sit, and Sit+3MA groups by immunofluorescence staining for LC3II (scale bar: 20 µm). (B) Histogram of fluorescence intensity of LC3II-positive cells in the dermal layer in each group. (C) IHC staining of CTSD expression in the dermis (original magnification: 200×; scale bar: 50 µm). (D) Histogram of CTSD level estimated by IHC. (E) Western blotting for Beclin1, VPS34, CTSD, SQSTM1/p62, and LC3II expressions in flap of the control, Sit, and Sit+3MA groups. All gels have been run under the same electrophoretic conditions and cropped blots are used here. (F) Histogram of autophagy related protein expressions of Beclin1, p62, CTSD, VPS34, and LC3II calculated as the folds of control. **p < 0.01, vs control group; @@p < 0.01, vs Sit group. Data are presented as mean ± standard error, n = 6 per group.