Figure 5. Cholesterol content and glucose uptake are tightly linked in ECs.

1. Measurement of cellular cholesterol content (upper panel) and glucose uptake (lower panel) in HUVECs treated with VEGF‐B₁₈₆ for 1 h or after 15‐min treatment with cholesterol‐extracting methyl‐beta‐cyclodextrin (MbCD). Data are presented as mean ± SEM from a representative experiment performed in triplicates. Statistical evaluation using one‐way ANOVA and Dunnett's multiple comparison test, P‐value: ***< 0.001 (compared to untreated control).
2. Measurement of cellular cholesterol content (upper panel) and glucose uptake (lower panel) in HUVECs treated with VEGF‐B₁₈₆ for 15 min alone or supplemented with soluble methyl‐beta‐cyclodextrin complexed with cholesterol (5, 10 or 20 μg/ml cholesterol). Data presented as mean ± SEM from a representative experiment performed in triplicates. Statistical evaluation using one‐way ANOVA and Dunnett's multiple comparison test, P‐value: *< 0.05, **< 0.01, ***< 0.001 (compared to control w/o cholesterol).

Data information: See also Fig EV5.