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. 2020 May 24;21(7):e49343. doi: 10.15252/embr.201949343

Figure 7. VEGF‐B regulates cardiac cholesterol content in vivo .

Figure 7

  1. Quantification of non‐esterified cholesterol content by means of filipin staining in the blood vessel compartment (podocalyxin+) in hearts of 15‐week‐old male C57BL/6 Vegfb +/− (n = 5) and Vegfb −/− mice (n = 7), normalized to wild‐type (Vegfb +/+) mice (n = 8). Data presented as box‐and‐whisker plots. Boxes represent lower/upper quartiles with the median values indicated with a horizontal line. Whiskers represent min–max values. Statistical evaluation using one‐way ANOVA and Dunnett's multiple comparison test, P‐value: *< 0.05, **< 0.01 (compared to Vegfb +/+ mice).
  2. Quantification of non‐esterified cholesterol content by means of filipin staining in hearts of 15‐week‐old male C57BL/6 Vegfb +/− (n = 5) and Vegfb −/− (n = 7) mice, normalized to wild‐type (Vegfb +/+) mice (n = 8). Data presented as box‐and‐whisker plots. Boxes represent lower/upper quartiles with the median values indicated with a horizontal line. Whiskers represent min–max values. Statistical evaluation using one‐way ANOVA and Dunnett's multiple comparison test, P‐value: **< 0.01 (compared to Vegfb +/+ mice).
  3. Quantification of non‐esterified cholesterol content by means of filipin staining in hearts of 25‐week‐old male CD1 Flt1 +/lacz (n = 20) mice, normalized to wild‐type CD1 (Flt1 +/+) mice (n = 16). Data presented as box‐and‐whisker plots. Boxes represent lower/upper quartiles with the median values indicated with a horizontal line. Whiskers represent min–max values. Statistical evaluation using t‐test, P‐value: **< 0.01.
  4. Quantification of non‐esterified cholesterol content by means of filipin staining in hearts of 12‐ to 16‐week‐old male C57BL/6 wild‐type (Vegfb +/+) (n = 4), BKS.C57BL/6 db/+ Vegfb +/+ (n = 8), db/db Vegfb +/− (n = 10), and db/db Vegfb −/− (n = 4) mice, normalized to db/db Vegfb +/+ (n = 6) mice. Data presented as box‐and‐whisker plots. Boxes represent lower/upper quartiles with the median values indicated with a horizontal line. Whiskers represent min–max values. Statistical evaluation using one‐way ANOVA and Dunnett's multiple comparison test, P‐value: *< 0.05, ***< 0.001 (compared to db/db Vegfb +/+ mice).
  5. Left panel: Quantification of non‐esterified cholesterol content by means of filipin staining in hearts of C57BL/6 male mice after 25 weeks on HFD, long‐term treated with isotype control (n = 17) or VEGF‐B blocking antibody (2H10, n = 17) initiated after 5 weeks on HFD, normalized to age‐matched mice on chow diet (n = 10). Data presented as box‐and‐whisker plots. Boxes represent lower/upper quartiles with the median values indicated with a horizontal line. Whiskers represent min–max values. Right panel: Body weights (mean ± SEM) of HFD (n = 17+17) and chow fed littermate mice (n = 10). Statistical evaluation using one‐way ANOVA and Dunnett's multiple comparison test, P‐value: *< 0.05, ***< 0.001 (compared to HFD control mice).
  6. Quantification of non‐esterified cholesterol content by means of filipin staining in hearts of mice on HFD for 17 weeks followed by treatment for 1 week with 2H10 antibody (n = 5) compared to control antibody treated mice (n = 5). Data presented as box‐and‐whisker plots. Boxes represent lower/upper quartiles with the median values indicated with a horizontal line. Whiskers represent min–max values. Statistical evaluation using t‐test, P‐value: **< 0.01.
Data information: See also Appendix Fig S6.