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. 2020 Apr 30;21(7):e49666. doi: 10.15252/embr.201949666

Figure EV2. EphA2 has no effect on IL‐1β production in BMDMs in response to reovirus.

Figure EV2

  1. ELISA of IL‐1β in the supernatant of BMDM cells from wild‐type (WT) and EphA2‐knockout (KO) mice (n = 4 per strain) stimulated for 16 h with the RNA virus reovirus (MOI = 5).
  2. Immunoblot analysis of EphA2 in lysates of the RAW264.7 cell line; β‐actin served as a loading control throughout. Cells were transfected with HA vector or HA‐EphA2 plasmid.
  3. ELISA of IL‐1β in the supernatant of the RAW264.7 cells transfected with HA vector or HA‐EphA2 plasmid and stimulated for 16 h with the RNA virus reovirus (MOI = 5).
  4. ELISA of IL‐1β in the supernatant of AECs from wild‐type (WT) and EphA2‐knockout (KO) mice (n = 4 per strain) treated with LPS (200 ng/ml, 4 h) and nigericin (7.5 mM, 1 h).
Data information: Each symbol represents an independent experiment; small horizontal lines indicate the average of triplicates. ***P < 0.001 (unpaired t‐test). NS means no statistical difference. Data represent four independent biological replicates.Source data are available online for this figure.