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. 2020 Apr 30;21(7):e49666. doi: 10.15252/embr.201949666

Figure 4. EphA2 functions as a negative regulator in ova‐induced airway inflammation.

Figure 4

  • A, B
    Quantitative RT–PCR analysis of mRNA encoding various cytokines (IL‐4, IL‐5, IL‐13, IL‐33) in lung samples from wild‐type (WT) and EphA2‐knockout (KO) mice (n = 4 per strain) sensitized with ova; results presented relative to β‐actin. Shown is mean ± SD.
  • C
    ELISA of IL‐33 in lung tissue homogenate samples from wild‐type (WT) and EphA2‐knockout (KO) mice (n = 3 per strain) sensitized with ova.
  • D–G
    ELISA of IL‐4, IL‐13, IL‐1β, and IL‐18 in lung tissue homogenate samples from wild‐type (WT) and EphA2‐knockout (KO) mice (n = 3 per strain) sensitized with ova.
  • H
    Cell number and category of BALF samples from wild‐type (WT) and EphA2‐knockout (KO) mice (n = 3 per strain) sensitized with ova. Shown is mean ± SD.
  • I
    H&E staining and PAS staining of lung sections from wild‐type (WT) and EphA2‐knockout (KO) mice sensitized with ova. Scale bars represent 200 μm.
Data information: Each symbol represents an independent experiment; small horizontal lines indicate the average of triplicates. *P < 0.05, **P < 0.01, and ***P < 0.001 (unpaired t‐test). Data represent three or four independent biological replicates.