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A, B
Confocal microscopy analysis of AECs uninfected or infected with reovirus (MOI = 20) for 3 h. AECs primed with LPS and stimulated with ATP for 30 min severed as a positive control. ASC was stained with mouse anti‐ASC (1:200), followed by Alexa Fluor 488 goat anti‐mouse secondary antibody (green). DAPI served as the nuclei marker (blue).
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C, D
Confocal microscopy analysis of HEK293T cells transfected with Flag‐ASC and Myc‐tagged vector or Myc‐tagged NLRP3 or Myc‐tagged NLRP3 Y132F. Cells were stained with anti‐FLAG (1:200) and anti‐Myc (1:200), followed by Alexa Fluor 488 goat anti‐rabbit secondary antibody (green) or Alexa Fluor 594 goat anti‐mouse secondary antibody (red). DAPI served as the nuclei marker (blue).
Data information: Scale bars represent 50 μm. Each symbol represents an independent experiment; small horizontal lines indicate the average of triplicates. **
P < 0.01 and ***
P < 0.001 (unpaired
t‐test). Data represent three independent biological replicates.
