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. 2020 May 8;21(7):e49762. doi: 10.15252/embr.201949762

Table 1.

Primer sequences used for qPCR

Gene Forward primer (5′–3′) Reverse primer (5′–3′)
Actin GCTTCTAGGCGGACTGTTACTGA GCCATGCCAATGTTGTCTCTTAT
Rpl CCTGCTGCTCTCAAGGTT TGGTTGTCACTGCCTGGTACTT
Ubc AGGTCAAACAGGAAGACAGACGTA TCACACCCAAGAACAAGCACA
Gapdh TGAAGCAGGCATCTGAGGG CGAAGGTGGAAGAGTGGGAG
Ifit1 CTGAGATGTCACTTCACATGGAA GTGCATCCCCAATGGGTTCT
Irf8 AGACCATGTTCCGTATCCCCT CACAGCGTAACCTCGTCTTCC
Irf1 ATGGAAACCCCGAAACCGC GATATTTCCAGTGGCCTGGA
Stat1 TCACAGTGGTTCGAGCTTCAG GCAAACGAGACATCATAGGCA
Ripk3 GTGCTACCTACACAGCTTGGA CCCTCCCTGAAACGTGGAC
Mlkl AATTGTACTCTGGGAAATTGCCA TCTCCAAGATTCCGTCCACAG
Zbp1 AAGAGTCCCCTGCGATTATTTG TCTGGATGGCGTTTGAATTGG
Defa6 CCAGGCTGATCCTATCCAAA GTCCCATTCATGCGTTCTCT
Lyz1 ATGGAATGGCTGGCTACTATGG ACCAGTATCGGCTATTGATCTGA