Figure 5. CDF4 suppresses H₂O₂ scavenging.

• A
  The senescence phenotype of the vector control, pER8::CDF4, and pER8::CDF4::RNAi detached leaves treated with H₂O₂ and incubated in the dark for 3 days. The third leaf in rosettes of 3‐week‐old plants was detached and incubated in MES buffer (2 mM MES, pH 5.8) treated with 2 mM H₂O₂ and 20 μM β‐estradiol under dark conditions for 3 days. Picture shows the leaves before and after treatment. Scale bars indicate 1 cm.
• B, C
  (B) DAB staining and (C) measurement of H₂O₂ before and after 2 mM H₂O₂ plus 20 μM β‐estradiol treatment under dark conditions for 2 days. The brown color represents H₂O₂ accumulation. Three independent experiments were conducted. Values are given as mean ± SD, n = 3. *P < 0.05 by Student's t‐test. Scale bars indicate 1 cm.
• D
  Chlorophyll concentrations in the leaves shown in (A). Three independent experiments were conducted. Values are given as mean ± SD, n = 3. *P < 0.05 by Student's t‐test.