REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Pharmaceutical-grade rigosertib	Onconova Therapeutics, Inc	N/A
ON01500	Onconova Therapeutics, Inc	N/A
Commercially obtained rigosertib	Selleck Chemicals	Cat#S1362
Experimental Models: Cell Lines
K562 CRISPRi	Gilbert et al., 2014	N/A
K562 CRISPRa	Gilbert et al., 2014	N/A
RPE1 CRISPRi with EB3-GFP	Jost et al., 2017	N/A
Oligonucleotides
See “Targeting Sequences of sgRNAs” table below for sgRNA sequences	Jost et al., 2017	N/A
Recombinant DNA
pHR-SFFV-HA-IRES-mCherry	Jost et al., 2017	N/A
pHR-SFFV-TUBB(L240F)-IRES-mCherry	Jost et al., 2017	N/A
Software and Algorithms
Micro-Manager microscope control software	Edelstein et al., 2014	https://micro-manager.org/